Host: Rabbit
Target Protein: EZH2
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 2146
Swiss Prot: Q15910
Source: KLH conjugated synthetic peptide derived from human EZH2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1mg/ml
Applications:
WB=1:500-2000
IHC-P=1:200-1000
ICC=1:50
IF=1:50
Predicted Molecular Weight: 82
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Lysate: HeLa, Jurkat, MCF7, Neuro-2a, 3T3 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 85 kDa Observed MW: 90 kDa
Tissue:Human cervical carcinoma Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60001R
Tissue:mouse spleen Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60001R
Tissue:mouse testis Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60001R
Tissue:rat spleen Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60001R
Lane 1: Mouse Brain Lysates; Lane 2: Mouse Cerebellum Lysates; Lane 3: Human MCF-7 cell Lysates; Lane 4: Human Hela cell Lysates; Lane 5: Human Jurkat cell Lysates; Lane 6: Human Jar cell Lysates; Lane 7: Human HL-60 cell Lysates; Lane 8: Human K562 cell Lysates; Lane 9: Human U251 cell Lysates. Probed with EZH2 monoclonal Antibody, unconjugated (bsm-60001R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.