Host: Rabbit
Target Protein: Glypican 3
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 2719
Swiss Prot: P51654
Source: A synthesized peptide derived from human Glypican 3
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: Lot dependent
Predicted Molecular Weight: 65
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: HepG2 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 66 kDa Observed MW: 66 kDa
25 ug total protein per lane of various lysates (see on figure) probed with Glypican 3 monoclonal antibody, unconjugated (bsm-60006R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.