bsm-60010R [Primary Antibody]
Insulin Recombinant Antibody
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Host: Rabbit

Target Protein: Insulin

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 3630

Swiss Prot: P01308

Source: KLH conjugated synthetic peptide derived from human Insulin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.

Size: 100µL

Concentration: 1mg/ml

Applications: IHC-P(IHC-P(1:1000-5000))
IHC-F(IHC-F(1:1000-5000))
IF(IHC-P)(IF(IHC-P)(1:1000-5000))

Predicted Molecular Weight: 12


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Insulin Monoclonal Antibody, Unconjugated(bsm-60010R) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Insulin Monoclonal Antibody, Unconjugated(bsm-60010R) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Insulin Monoclonal Antibody, Unconjugated(bsm-60010R) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.