bsm-60050M [Primary Antibody]
Acetyl-Histone H3 (Lys18) Monoclonal Antibody
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Host: Mouse

Target Protein: Acetyl-Histone H3 (Lys18)

Clonality: Monoclonal

Isotype: IgG

Swiss Prot: P68431

Purification: Protein G coupled with the antigen peptide containing acetylated H3 (Lys18).

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Size: 100µl

Concentration: Lot Dependent

Applications: WB
IHC-F
IF
IHC-P
ICC/IF

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 17 kDa Observed MW: 17 kDa


Tissue: Human neuroblastoma Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:200 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Mouse)(sp-0024) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60050M