bsm-60050M [Primary Antibody]
Acetyl-Histone H3 (Lys18) Monoclonal Antibody
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DATASHEET

Host: Mouse

Target Protein: Acetyl-Histone H3 (Lys18)

Clonality: Monoclonal

Isotype: IgG

Swiss Prot: P68431

Purification: Protein G coupled with the antigen peptide containing acetylated H3 (Lys18).

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Size: 100ul

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-F(1:50-200)
IF(1:50-200)
IHC-P(1:50-200)
ICC/IF(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Acetyl-Histone H3 (Lys18)) monoclonal Antibody, unconjugated (bsm-60050M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.


25 ug total protein per lane of various lysates (see on figure) probed with H3C1 | Histone H3 (ac-K18) monoclonal antibody, unconjugated (bsm-60050M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H3C1 | Histone H3 (ac-K18) Monoclonal Antibody, Unconjugated (bsm-60050M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H3C1 | Histone H3 (ac-K18) Monoclonal Antibody, Unconjugated (bsm-60050M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H3C1 | Histone H3 (ac-K18) Monoclonal Antibody, Unconjugated (bsm-60050M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H3C1 | Histone H3 (ac-K18) Monoclonal Antibody, Unconjugated (bsm-60050M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H3C1 | Histone H3 (ac-K18) Monoclonal Antibody, Unconjugated (bsm-60050M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H3C1 | Histone H3 (ac-K18) Monoclonal Antibody, Unconjugated (bsm-60050M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H3C1 | Histone H3 (ac-K18) Monoclonal Antibody, Unconjugated (bsm-60050M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H3C1 | Histone H3 (ac-K18) Monoclonal Antibody, Unconjugated (bsm-60050M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H3C1 | Histone H3 (ac-K18) Monoclonal Antibody, Unconjugated (bsm-60050M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H3C1 | Histone H3 (ac-K18) Monoclonal Antibody, Unconjugated (bsm-60050M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H3C1 | Histone H3 (ac-K18) Monoclonal Antibody, Unconjugated (bsm-60050M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.