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Host: Mouse
Target Protein: Acetyl-Histone H4 (Lys5)
Clonality: Monoclonal
Isotype: IgG
Swiss Prot: P62805
Purification: Protein G coupled with the antigen peptide containing acetylated H4 (Lys5).
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: Lot Dependent
Applications:
WB(1:300-5000)
IHC-F(1:50-200)
IF(1:50-200)
IHC-P(1:50-200)
ICC/IF(1:50-200)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Acetyl-Histone H4 (Lys5)) monoclonal Antibody, unconjugated (bsm-60053M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.