Host: Mouse
Target Protein: Histone H2B (Acetyl K11)
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 3018
Swiss Prot: P33778
Source: KLH conjugated synthetic peptide derived from human Histone H2B (Acetyl K11)
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1mg/ml
Applications:
WB=1:500-2000
Predicted Molecular Weight: 14
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species: Rat
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 14 kDa Observed MW: 14 kDa
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:500 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: Mouse liver, Mouse spleen Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 14 kDa Observed MW: 14 kDa