bsm-60066M [Primary Antibody]
Acetyl-Histone H4 (Lys5/8/12/16) Monoclonal Antibody
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DATASHEET

Host: Mouse

Target Protein: Acetyl-Histone H4 (Lys5/8/12/16)

Clonality: Monoclonal

Isotype: IgG

Swiss Prot: P68431

Purification: Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Size: 100µl

Concentration: Lot Dependent

Applications: WB
IHC-F
IF
IHC-P
ICC/IF

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

C2C12 (M) treated with Trichostatin A (TSA) (1μM) for 18 h, HeLa (H) treated with Trichostatin A (TSA) (1μM) for 18 h, 25 μg total protein per lane of cell lysates (see on figure) probed with Acetyl-Histone H4 (Lys5/8/12/16) monoclonal antibody, unconjugated (bsm-60066M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys5/8/12/16) Monoclonal Antibody, Unconjugated (bsm-60066M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys5/8/12/16) Monoclonal Antibody, Unconjugated (bsm-60066M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys5/8/12/16) Monoclonal Antibody, Unconjugated (bsm-60066M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys5/8/12/16) Monoclonal Antibody, Unconjugated (bsm-60066M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys5/8/12/16) Monoclonal Antibody, Unconjugated (bsm-60066M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys5/8/12/16) Monoclonal Antibody, Unconjugated (bsm-60066M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys5/8/12/16) Monoclonal Antibody, Unconjugated (bsm-60066M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys5/8/12/16) Monoclonal Antibody, Unconjugated (bsm-60066M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys5/8/12/16) Monoclonal Antibody, Unconjugated (bsm-60066M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys5/8/12/16) Monoclonal Antibody, Unconjugated (bsm-60066M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys5/8/12/16) Monoclonal Antibody, Unconjugated (bsm-60066M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.