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Host: Mouse
Target Protein: Phospho-Histone H3 (Ser28)
Clonality: Monoclonal
Isotype: IgG2b/Kappa
Swiss Prot: P68431
Purification: Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications: WB(1:300-5000)
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: (-) HeLa, (+) HeLa+Nocodazole (100ng/ml, 18hr) Protein loading quantity: 20 μg Exposure time: 10 s Predicted MW: 15 kDa Observed MW: 15 kDa