bsm-60080M [Primary Antibody]
Phospho-Histone H3 (Ser10) Monoclonal Antibody
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DATASHEET

Host: Mouse

Target Protein: Phospho-Histone H3 (Ser10)

Clonality: Monoclonal

Isotype: IgG1/kappa

Swiss Prot: P68431

Purification: Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Size: 100ul

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-F(1:50-200)
IF(1:50-200)
IHC-P(1:50-200)
ICC/IF(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

HeLa (H) treated with Calyculin A (100nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-Histone H3 (Ser10) monoclonal antibody, unconjugated (bsm-60080M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3 (Ser10) Monoclonal Antibody, Unconjugated (bsm-60080M) at 1:200 overnight at 4°C, followed by conjugation to bs-40296G-HRP and DAB (C-0010) staining.


NIH/3T3 (M) + Serum (0%, overnight) add Nocodazole (100ng/mL) for 18 h, add Calyculin A (100ng/mL) for 1 h, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-Histone H3 (Ser10) monoclonal antibody, unconjugated (bsm-60080M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3 (Ser10) Monoclonal Antibody, Unconjugated (bsm-60080M) at 1:200 overnight at 4°C, followed by conjugation to bs-40296G-HRP and DAB (C-0010) staining.


C6 (R) treated with Calyculin A (100nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-Histone H3 (Ser10) monoclonal antibody, unconjugated (bsm-60080M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3 (Ser10) Monoclonal Antibody, Unconjugated (bsm-60080M) at 1:200 overnight at 4°C, followed by conjugation to bs-40296G-HRP and DAB (C-0010) staining.