Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: lane 1: PTM-5003+H3K9me2 peptides, lane 2: PTM-5003+unmodified peptides, lane 3: PTM-5003, 2 hours at room temperature Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: HeLa Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 15 kDa Observed MW: 15 kDa


Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: HeLa, N2a, BRL, Mouse Liver, Mouse kidney Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 15 kDa Observed MW: 15 kDa


Tissue: Human neuroblastoma Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:200 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Mouse)(sp-0024) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60083M