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Host: Mouse
Target Protein: Mono-Methyl-Histone H2B (Lys15)
Clonality: Monoclonal
Isotype: IgG
Swiss Prot: P62807
Purification: Protein G coupled with the antigen peptide containing H2BK15me1.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications: WB(1:300-5000)
Predicted Molecular Weight: 14
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Monkey
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: HeLa, C2C12, COS-7, BRL Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 14 kDa Observed MW: 14 kDa
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: Mouse spleen, Mouse liver Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 14 kDa Observed MW: 14 kDa