bsm-60167R [Primary Antibody]
Ubiquityl-Histone H2B (Lys120) Recombinant Antibody
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Host: Rabbit

Target Protein: Ubiquityl-Histone H2B (Lys120)

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 3018

Swiss Prot: P33778

Source: KLH conjugated synthetic peptide derived from human Ubiquityl-Histone H2B (Lys120)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008].

Size: 100uL, 20uL

Concentration: Lot Dependent

Applications: WB(WB=1:500-2000)
IHC-P(IHC-P=1:200-500)
IHC-F(1:100-500)
IF(1:50-200)

Predicted Molecular Weight: 14


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ubiquityl-Histone H2B (Lys120) Monoclonal Antibody, Unconjugated (bsm-60167R) at 1:150 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.