VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: 2 hours at room temperature.
Lysate: Rat skin, Mouse skin
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 52 kDa
Observed MW: 52 kDa
Tissue: Human breast
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for bsm-60225R
Tissue: Human esophagus
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for bsm-60225R
Tissue: Human prostate hyperplasia
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for bsm-60225R