bsm-60233R [Primary Antibody]
CD22 Recombinant Antibody
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Host: Rabbit

Target Protein: CD22

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 933

Swiss Prot: P20273

Source: KLH conjugated synthetic peptide derived from human CD22

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Predicted to enable CD4 receptor binding activity; protein phosphatase binding activity; and sialic acid binding activity. Involved in B cell activation; negative regulation of B cell receptor signaling pathway; and regulation of endocytosis. Located in early endosome and recycling endosome. [provided by Alliance of Genome Resources, Nov 2021]

Size: 100µL

Concentration: Lot dependent

Applications: WB=1:500-2000
IHC-P=1:400-800
ICC=1:100
IF=1:100-500

Predicted Molecular Weight: 91


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD22 Monoclonal Antibody, Unconjugated(bsm-60233R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD22 Monoclonal Antibody, Unconjugated(bsm-60233R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with CD22 monoclonal antibody, unconjugated (bsm-60233R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.