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Host: Rabbit
Target Protein: BOB-1
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 5450
Swiss Prot: Q16633
Source: KLH conjugated synthetic peptide derived from human BOB-1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100µL
Concentration: 1mg/ml
Applications:
WB=1:500-1000
IHC-P=1:200-1:1000
ICC=1:50-100
IF=1:50-100
Predicted Molecular Weight: 28
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Lysate: Ramos, Daudi, Raji Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 27 kDa Observed MW: 35 kDa
Tissue: Human lymphoma Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60269R