bsm-60286R [Primary Antibody]
LYPD3 Recombinant Antibody
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Host: Rabbit

Target Protein: LYPD3

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 27076

Swiss Prot: O95274

Source: KLH conjugated synthetic peptide derived from human LYPD3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Predicted to enable laminin binding activity. Predicted to act upstream of or within cell-matrix adhesion. Located in extracellular space. [provided by Alliance of Genome Resources, Nov 2021]

Size: 100µL

Concentration: Lot Dependent

Applications: WB(WB=1:500-1000)
IHC-P(IHC-P=1:50-100)
IHC-F(ICC=1:50)
IF(IF=1:50-100)
ICC/IF(1:50-100)

Predicted Molecular Weight: 33


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Lysate: A431 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 36 kDa Observed MW: 70 kDa


Tissue: Human tonsil Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60286R


Tissue: Human skin Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60286R


Tissue: Human esophagus Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60286R