bsm-60313R [Primary Antibody]
CD2 Recombinant Antibody
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Host: Rabbit

Target Protein: CD2

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 914

Swiss Prot: P06729

Source: KLH conjugated synthetic peptide derived from human CD2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

CD2 is a surface antigen of the human T lymphocyte lineage that is expressed on all peripheral blood T cells. It is one of the earliest T cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes.
CD2 interacts with lymphocyte function associated antigen (LFA3) and CD48/BCM1 to mediate adhesion between T cells and other cell types. CD2 is implicated in the triggering of T cells, the cytoplasmic domain is implicated in the signaling function. It is useful for the identification of lymphomas and leukaemias of T cell origin.

Size: 100µL

Concentration: Lot Dependent

Applications: IHC-P(ICC=1:50)
IHC-F(IF=1:50)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 37


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD2 Monoclonal Antibody, Unconjugated(bsm-60313R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD2 Monoclonal Antibody, Unconjugated(bsm-60313R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD2 Monoclonal Antibody, Unconjugated(bsm-60313R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD2 Monoclonal Antibody, Unconjugated(bsm-60313R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD2 Monoclonal Antibody, Unconjugated(bsm-60313R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD2 Monoclonal Antibody, Unconjugated(bsm-60313R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD2 Monoclonal Antibody, Unconjugated(bsm-60313R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD2 Monoclonal Antibody, Unconjugated(bsm-60313R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.