bsm-60328R [Primary Antibody]
SIGLEC1 Recombinant Antibody
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Host: Rabbit

Target Protein: SIGLEC1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 6614

Swiss Prot: Q9BZZ2

Source: KLH conjugated synthetic peptide derived from human Sialoadhesin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:200-1000
ICC=1:50
IF=1:50

Predicted Molecular Weight: 186


Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SIGLEC1 Monoclonal Antibody, Unconjugated (bsm-60328R) at 1:300 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SIGLEC1 Monoclonal Antibody, Unconjugated (bsm-60328R) at 1:300 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SIGLEC1 Monoclonal Antibody, Unconjugated (bsm-60328R) at 1:300 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.