Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4°C overnight Lysate: 1: HeLa, 2: HEK-293, 3: HCT-116, 4: RAW264.7 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 61 kDa Observed MW: 61 kDa


Tissue: Human brain Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60333M


Tissue: Human breast cancer Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60333M