DATASHEET
Host:
Mouse
Target Protein:
CYP11A1
Clonality:
Monoclonal
Isotype:
IgG1/Kappa
Entrez Gene:
1583
Swiss Prot:
P05108
Source:
KLH conjugated synthetic peptide derived from human CYP11A1
Purification:
Purified by Protein G.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]
VALIDATION IMAGES
Tissue: Human placenta Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab:SP Kit(Mouse)(sp-0024) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60352M
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: room temperature 2h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: JAR, HepG2
Protein loading quantity: 20 μg
Exposure time: 3 s
Predicted MW: 52 kDa
Observed MW: 52 kDa