Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition:4°C overnight Lysate: HeLa, HepG2, 293, HT-29 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 31 kDa Observed MW: 31 kDa


Tissue: Human breast cancer Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60362M


Tissue: Human liver Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60362M