DATASHEET
Host:
Mouse
Target Protein:
CNPase
Clonality:
Monoclonal
Isotype:
IgG1/Kappa
Entrez Gene:
1267
Swiss Prot:
P09543
Source:
KLH conjugated synthetic peptide derived from human CNPase
Purification:
Purified by Protein G.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Predicted to enable 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity. Involved in substantia nigra development. Located in several cellular components, including extracellular space; microtubule; and plasma membrane. Implicated in hypomyelinating leukodystrophy 20; multiple sclerosis; and schizophrenia. Biomarker of alcoholic liver cirrhosis; multiple sclerosis; and restless legs syndrome. [provided by Alliance of Genome Resources, Nov 2021]
VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 4°C overnight
Lysate:1: HeLa, 2: HEK-293, 3: Rat cerebellum
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 48 kDa
Observed MW: 48 kDa
Lane 1: Rat Cerebrum tissue lysates; Lane 2: Rat Cerebellum tissue lysates probed with CNPase Monoclonal Antibody, Unconjugated (bsm-60368M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.