bsm-60368M [Primary Antibody]
CNPase monoclonal Antibody
www.biossusa.com
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DATASHEET

Host: Mouse

Target Protein: CNPase

Clonality: Monoclonal

Isotype: IgG1/Kappa

Entrez Gene: 1267

Swiss Prot: P09543

Source: KLH conjugated synthetic peptide derived from human CNPase

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Predicted to enable 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity. Involved in substantia nigra development. Located in several cellular components, including extracellular space; microtubule; and plasma membrane. Implicated in hypomyelinating leukodystrophy 20; multiple sclerosis; and schizophrenia. Biomarker of alcoholic liver cirrhosis; multiple sclerosis; and restless legs syndrome. [provided by Alliance of Genome Resources, Nov 2021]

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000
ICC=1:50
IF=1:100-500

Predicted Molecular Weight: 48


Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 4°C overnight Lysate:1: HeLa, 2: HEK-293, 3: Rat cerebellum Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 48 kDa Observed MW: 48 kDa


Lane 1: Rat Cerebrum tissue lysates; Lane 2: Rat Cerebellum tissue lysates probed with CNPase Monoclonal Antibody, Unconjugated (bsm-60368M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.