bsm-60374M [Primary Antibody]
14-3-3 Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: 3/3/14

Clonality: Monoclonal

Isotype: IgG1/Kappa

Swiss Prot: P27348

Purification: Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:300-5000)
ICC(1:100-500)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: 1: HeLa, 2: A549, 3: Neuro-2a, 4: PC-12 Protein loading quantity: 20 μg Exposure time: 10 s Predicted MW: 28 kDa Observed MW: 29 kDa