bsm-60378M
[Primary Antibody]
brachyury Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: brachyury
Clonality: Monoclonal
Isotype: IgG1/Kappa
Swiss Prot: O15178
Purification: Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications: WB(1:300-5000)
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2500 Primary ab incubation condition: 4°C overnight Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: 1: A549, 2: HeLa, 3: Jurkat Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 47 kDa Observed MW: 49 kDa