bsm-60390R [Primary Antibody]
CD3G Recombinant Antibody
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Host: Rabbit

Target Protein: CD3G

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 917

Swiss Prot: P09693

Source: KLH conjugated synthetic peptide derived from human CD3G

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq, Jul 2008]

Size: 100µL

Concentration: Lot Dependent

Applications: WB(WB=1:500-1000)
IHC-P(IHC-P=1:500-2000)
IHC-F(ICC=1:50)
IF(IF=1:50)
ICC/IF(1:50-100)

Predicted Molecular Weight: 20


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: Jurkat Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 20 kDa Observed MW: 18-28 kDa


Paraformaldehyde-fixed, paraffin embedded Human Pyogenic appendicitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD3G Monoclonal Antibody, Unconjugated (bsm-60390R) at 1:300 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Lane 1: Human Jurkat cell Lysates; Lane 2: Human MOLT4 cell Lysates. Probed with CD3G monoclonal Antibody, unconjugated (bsm-60390R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.