bsm-60400M [Primary Antibody]
NRF1 monoclonal Antibody
www.biossusa.com
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DATASHEET

Host: Mouse

Target Protein: NRF1

Clonality: Monoclonal

Isotype: IgG2b/Kappa

Entrez Gene: 4899

Swiss Prot: Q16656

Source: KLH conjugated synthetic peptide derived from human NRF1

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014]

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000
ICC=1:100
IF=1:100-500

Predicted Molecular Weight: 55


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: 1: HeLa, 2: HEK-293, 3: Jurkat Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 54 kDa Observed MW: 70 kDa


Lane 1: Human Hela cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Human 293T cell lysates; Lane 5: Human Jurkat cell lysates probed with NRF1 monoclonal Antibody, Unconjugated (bsm-60400M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.