DATASHEET
Host:
Mouse
Target Protein:
NRF1
Clonality:
Monoclonal
Isotype:
IgG2b/Kappa
Entrez Gene:
4899
Swiss Prot:
Q16656
Source:
KLH conjugated synthetic peptide derived from human NRF1
Purification:
Purified by Protein G.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014]
VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: HeLa, 2: HEK-293, 3: Jurkat
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 54 kDa
Observed MW: 70 kDa
Lane 1: Human Hela cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Human 293T cell lysates; Lane 5: Human Jurkat cell lysates probed with NRF1 monoclonal Antibody, Unconjugated (bsm-60400M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.