bsm-60415M
[Primary Antibody]
peroxiredoxin 2 Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: peroxiredoxin 2
Clonality: Monoclonal
Isotype: IgG1/Kappa
Swiss Prot: P32119
Purification: Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(1:300-5000)
ICC(1:100-500)
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 4°C overnight Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: HeLa, F9, Mouse testis, PC-3 Protein loading quantity: 20 μg Exposure time: 3 s Predicted MW: 22 kDa Observed MW: 22 kDa
Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Mouse Cerebrum tissue lysates; Lane 5: Human Raji cell lysates; Lane 6: Human MCF-7 cell lysates; Lane 7: Human HeLa cell lysates; Lane 8: Human 293T cell lysates; Lane 9: Human MDA-MB-231 cell lysates; Lane 10: Human HepG2 cell lysates probed with PRDX2 Monoclonal Antibody, Unconjugated (bsm-60415M) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.