bsm-60432R [Primary Antibody]
TSHB Recombinant Antibody
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Host: Rabbit

Target Protein: TSHB

Clonality: Recombinant

Isotype: IgG

Swiss Prot: P01222

Purification: Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Size: 100µL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with TSHB monoclonal antibody, unconjugated (bsm-60432R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Rat pituitary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TSHB Monoclonal Antibody, Unconjugated (bsm-60432R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.