bsm-60433R
[Primary Antibody]
CLDN1 Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CLDN1
Clonality: Monoclonal
Isotype: IgG
Swiss Prot: O95832
Purification: Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Lechen Wang. et al. pH Sensitive Quercetin Nanoparticles Ameliorate DSS-Induced Colitis in Mice by Colon-Specific Delivery. MOL NUTR FOOD RES. 2023 Nov;:2300051Read more>>
- Li Lei. et al. Long-term Cu exposure alters CYP450s activity and induces jejunum injury and apoptosis in broilers. BIOMETALS. 2023 Nov;:1-12Read more>>
- Xinyun Qin. et al. Regulation of the intestinal flora using polysaccharides from Callicarpa nudiflora Hook to alleviate ulcerative colitis and the molecular mechanisms involved. INT J BIOL MACROMOL. 2024 Feb;258:128887Read more>>
VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HepG2, 2: A431, 3: Mouse liver Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 22 kDa Observed MW: 18 kDa
Tissue: Human colon cancer Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60433R
Tissue: Human esophagus Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60433R