bsm-60436M
[Primary Antibody]
INTS3 Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: INTS3
Clonality: Monoclonal
Isotype: IgG2b/Kappa
Swiss Prot: Q68E01
Purification: Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications: WB(1:300-5000)
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: 1: HeLa, 2: HEK-293, 3: MCF7, 4: RAW 264.7 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 118 kDa Observed MW: 118 kDa
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: lane 1: PTM-5729+competitive peptide, lane 2: PTM-5729, 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: C6 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 19 kDa Observed MW: 19 kDa