bsm-60451M
[Primary Antibody]
CAPG Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: CAPG
Clonality: Monoclonal
Isotype: IgG1/Kappa
Swiss Prot: P40121
Purification: Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(1:2000-10000)
ICC(1:100-500)
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Song Runjie. et al. A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer. MOL CANCER. 2023 Dec;22(1):1-2Read more>>
VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: room temperature 2h Secondary Ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: 1: HeLa, 2: Jurkat, 3: Raw264.7 Protein loading quantity: 20 μg Exposure time: 3 s Predicted MW: 40 kDa Observed MW: 40 kDa
Lane 1: Mouse Raw264.7 cell lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human Jurkat cell lysates probed with CAPG Monoclonal Antibody, Unconjugated (bsm-60451M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Lane 1: Mouse Raw264.7 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human Jurkat cell lysates probed with CAPG Monoclonal Antibody, Unconjugated (bsm-60451M) at 1:10000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.