Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:5000 Primary ab incubation condition: room temperature 2h Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate:1: HeLa, 2: 293, 3: BRL Protein loading quantity: 20 μg Exposure time: 3 s Predicted MW: 37 kDa Observed MW: 47 kDa


Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa whole cell lysate, using malonyl-histone H3 (Lys122) Rabbit mAb (PTM-910RM) pre-adsorbed with 3μM of the synthetic peptides; 2: HeLa whole cell lysate, using malonyl-histone H3 (Lys122) Rabbit mAb (PTM-910RM) with no peptide blocking. Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 15 kDa Observed MW: 15 kDa