bsm-60486M [Primary Antibody]
PSMB8 Monoclonal Antibody
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DATASHEET

Host: Mouse

Target Protein: PSMB8

Clonality: Monoclonal

Isotype: IgG1/kappa

Swiss Prot: P28062

Purification: Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Size: 100ul

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-F(1:50-200)
IF(1:50-200)
IHC-P(1:50-200)
ICC/IF(1:50-200)

Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with PSMB8 monoclonal antibody, unconjugated (bsm-60486M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PSMB8 Monoclonal Antibody, Unconjugated (bsm-60486M) at 1:100 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PSMB8 Monoclonal Antibody, Unconjugated (bsm-60486M) at 1:100 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PSMB8 Monoclonal Antibody, Unconjugated (bsm-60486M) at 1:100 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PSMB8 Monoclonal Antibody, Unconjugated (bsm-60486M) at 1:100 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PSMB8 Monoclonal Antibody, Unconjugated (bsm-60486M) at 1:100 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PSMB8 Monoclonal Antibody, Unconjugated (bsm-60486M) at 1:100 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human purulent appendicitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PSMB8 Monoclonal Antibody, Unconjugated (bsm-60486M) at 1:100 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.