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Host: Mouse
Target Protein: FIS1
Clonality: Recombinant
Isotype: IgG1/kappa
Entrez Gene: 51024
Swiss Prot: Q9Y3D6
Source: KLH conjugated synthetic peptide derived from human FIS1
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100µl
Concentration: Lot Dependent
Applications:
WB
IF(ICC)
Predicted Molecular Weight: 17
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
- Cheng-long Li. et al. Electroacupuncture protects against cerebral ischemia-reperfusion injury through mitochondrial dynamics. HELIYON. 2024 Jul;1Read more>>
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: room temperature 2h Secondary Ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: 1: MCF-7, 2: T47D, 3: MDA-MB-231 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa
Cell line: HepG2 Fixative: 4% Paraformaldehydel Permeabilization: 0.1% TritonX-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for bsm-60551M
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (FIS1) monoclonal Antibody, Unconjugated (bsm-60551M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.编号