bsm-60554R [Primary Antibody]
Aurora B Recombinant Antibody
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Host: Rabbit

Target Protein: Aurora B

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 9212

Swiss Prot: Q96GD4

Source: KLH conjugated synthetic peptide derived from human Aurora B

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromsomes 19 and 20. These kinases participate in the regulation of segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012].

Size: 100µL

Concentration: Lot Dependent

Applications: WB(WB=1:500-1000)
IHC-P(IHC-P=1:50-100)
IHC-F(ICC=1:50)
IF(IF=1:50)
ICC/IF(1:50-100)

Predicted Molecular Weight: 39


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: 293, 3: A20 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 39 kDa Observed MW: 39 kDa


Tissue: Human tonsil Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60554R


Tissue: mouse spleen Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60554R


Lane 1: Human Hela cell Lysates; Lane 2: Human 293T cell Lysates. Probed with Aurora B monoclonal Antibody, unconjugated (bsm-60554R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.