bsm-60573R
[Primary Antibody]
RBBP4 Recombinant Antibody
DATASHEET
Host: Rabbit
Target Protein: RBBP4
Clonality: Recombinant
Isotype: IgG/Kappa
Swiss Prot: Q09028
Purification: Protein G.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(WB=1:500-1:1000)
IHC-P(IHC-P=1:100-500)
IHC-F(IF=0)
IF()
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: HeLa,HEK- 293, A20, Rat spleen Protein loading quantity: 20 μg Exposure time: 3 s Predicted MW: 52 kDa Observed MW: 52 kDa
Tissue: Human lung squamous carcinoma Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60573R