Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: SHSY-5Y, 2: MOLT-4, 3: NIH-3T3, 4: C6, 5: Mouse testis, 6: Rat testis Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 47 kDa Observed MW: 65 kDa


Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: SHSY-5Y cell lysate, the SUV39H2 Rabbit mAb (PTM-5987) pre-adsorbed with 3μM of the synthetic peptides; 2: SHSY-5Y cell lysate, the SUV39H2 Rabbit mAb (PTM-5987) with no peptide blocking. Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 47 kDa Observed MW: 65 kDa