bsm-60609R [Primary Antibody]
CDK8 Recombinant Antibody
www.biossusa.com
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CDK8

Clonality: Monoclonal

Isotype: IgG

Swiss Prot: P49336

Purification: Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Size: 100µl

Concentration: Lot Dependent

Applications: WB
IHC-F
IF(IHC-P)
IHC-P

Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDK8 Monoclonal Antibody, Unconjugated (bsm-60609R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDK8 Monoclonal Antibody, Unconjugated (bsm-60609R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDK8 Monoclonal Antibody, Unconjugated (bsm-60609R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDK8 Monoclonal Antibody, Unconjugated (bsm-60609R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDK8 Monoclonal Antibody, Unconjugated (bsm-60609R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDK8 Monoclonal Antibody, Unconjugated (bsm-60609R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDK8 Monoclonal Antibody, Unconjugated (bsm-60609R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDK8 Monoclonal Antibody, Unconjugated (bsm-60609R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDK8 Monoclonal Antibody, Unconjugated (bsm-60609R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDK8 Monoclonal Antibody, Unconjugated (bsm-60609R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDK8 Monoclonal Antibody, Unconjugated (bsm-60609R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDK8 Monoclonal Antibody, Unconjugated (bsm-60609R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDK8 Monoclonal Antibody, Unconjugated (bsm-60609R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDK8 Monoclonal Antibody, Unconjugated (bsm-60609R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDK8 Monoclonal Antibody, Unconjugated (bsm-60609R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with CDK8 monoclonal antibody, unconjugated (bsm-60609R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.