bsm-60613R [Primary Antibody]
CD25 Recombinant Antibody
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Host: Rabbit

Target Protein: CD25

Clonality: Recombinant

Isotype: IgG

Swiss Prot: P01589

Purification: Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Size: 100µL

Concentration: 1mg/ml

Applications: WB(WB(1:500-2000))
FCM(FCM(1ug/Test))

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

The Jurkat (treated with 20ng/ml PMA for 24 hours) (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD25 antibody (bsm-60613R): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.


25 ug total protein per lane of various lysates (see on figure) probed with CD25 monoclonal antibody, unconjugated (bsm-60613R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.