bsm-60617R [Primary Antibody]
Ly6A Recombinant Antibody
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Host: Rabbit

Target Protein: Ly6A

Clonality: Monoclonal

Isotype: IgG

Swiss Prot: P05533

Purification: Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Size: 100µl

Concentration: Lot Dependent

Applications: WB
IHC-F
IF
IHC-P

Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Ly6A monoclonal antibody, unconjugated (bsm-60617R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ly6A Monoclonal Antibody, Unconjugated (bsm-60617R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ly6A Monoclonal Antibody, Unconjugated (bsm-60617R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.