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Host: Rabbit
Target Protein: CD68
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 968
Swiss Prot: P34810
Source: KLH conjugated synthetic peptide derived from human CD68
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
FCM(FCM(1ug/Test))
IHC-P(IHC-P(1:500-1000))
IHC-F(IHC-F(1:500-1000))
IF(IHC-P)(IF(IHC-P)(1:500-1000))
Predicted Molecular Weight: 37
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
- Xiaoyue Guan. et al. The Role of Macrophage Efferocytosis in the Pathogenesis of Apical Periodontitis. INT J MOL SCI. 2024 Jan;25(7):3854Read more>>
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Monoclonal Antibody, Unconjugated (bsm-60634R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Monoclonal Antibody, Unconjugated (bsm-60634R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD68 Monoclonal Antibody, Unconjugated (bsm-60634R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Monoclonal Antibody, Unconjugated (bsm-60634R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human endometrial carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Monoclonal Antibody, Unconjugated (bsm-60634R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
THP-1 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD68 Monoclonal Antibody(bsm-60634R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD68 Monoclonal Antibody, Unconjugated (bsm-60634R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.