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Host: Rabbit
Target Protein: Histone H2B (Crotonyl-Lys15 / Lys16 / Lys20)
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 3018
Swiss Prot: P33778
Source: KLH conjugated Synthesised acetylpeptide derived from human Histone H2B around the Crotonyl site of Lys15 / Lys16 / Lys20
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100uL
Concentration: Lot Dependent
Applications: WB(1:300-5000)
Predicted Molecular Weight: 14
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: (-): HeLa, (+): HeLa+ sodium butyrate (30mM, 4hr) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 14kDa Observed MW: 14kDa
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: (-): MCF-7, (+): MCF-7+ sodium butyrate (50mM, 24hr) + trichostain A (500ng/ml, 4 hr) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 14kDa Observed MW: 14kDa