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Host: Rabbit
Target Protein: phospho-SIRT1 (Ser27)
Modification Site: Ser27
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 23411
Swiss Prot: Q96EB6
Source: KLH conjugated synthetic peptide derived from human phospho-SIRT1 (Ser27)
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: Lot Dependent
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF
ICC/IF(1:50-100)
Predicted Molecular Weight: 81
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1-2: HEK-293+CA (-) (+), 3-4: Jurkat+CA(-) (+) Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 82 kDa Observed MW: 120 kDa
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-SIRT1 (Ser27) Monoclonal Antibody, Unconjugated (bsm-60654R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-SIRT1 (Ser27) Monoclonal Antibody, Unconjugated (bsm-60654R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-SIRT1 (Ser27) Monoclonal Antibody, Unconjugated (bsm-60654R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-SIRT1 (Ser27) Monoclonal Antibody, Unconjugated (bsm-60654R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-SIRT1 (Ser27) Monoclonal Antibody, Unconjugated (bsm-60654R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.