Host: Rabbit
Target Protein: phospho-TBK1 (Ser172)
Modification Site: Ser172
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 29110
Swiss Prot: Q9UHD2
Source: KLH conjugated synthetic peptide derived from human phospho-TBK1 (Ser172)
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Size: 100ul
Concentration: Lot dependent
Applications: WB(1:300-5000)
Predicted Molecular Weight: 84
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate:(-): HeLa, (+): HeLa+ Okadaic A+ Calyculin A (100 nM, 60min) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 84 kDa Observed MW: 84 kDa
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): C6, (+): C6+Calyculin A(100nM, 30min) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 84 kDa Observed MW: 84 kDa
Hela (H) cells were treated with or without Calyculin A (100nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-TBK1 monoclonal antibody, unconjugated (bsm-60659R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.