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Host: Rabbit
Target Protein: Histone H3 (Di-Methyl Lys122)
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 8350
Swiss Prot: P68431
Source: KLH conjugated synthetic peptide derived from human Histone H3 (Tri Methyl K27)
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100uL
Concentration: Lot Dependent
Applications: WB(1:300-5000)
Predicted Molecular Weight: 15
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species: Rat
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab:1: HeLa cytosol, 2: HeLa nuclear Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 15 KDa Observed MW: 15 KDa
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab:1: Mouse brain, 2: Rat brain, 3: Recombinant histone H3 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 15 KDa Observed MW: 15 KDa