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Host: Rabbit
Target Protein: phospho-eIF4EBP1 (Thr37 / Thr46)
Modification Site: Thr37 / Thr46
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 1978
Swiss Prot: Q13541
Source: KLH conjugated synthetic peptide derived from human phospho-eIF4EBP1 (Thr37 / Thr46)
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100uL
Concentration: Lot Dependent
Applications: WB(1:300-5000)
Predicted Molecular Weight: 13
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): HEK-293, (+): HEK-293 + EGF (200 ng/mL, 10 min) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 13 kDa Observed MW: 15 kDa