support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
Host: Rabbit
Target Protein: Histone H3 (Butyryl-Lys14)
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 8350
Swiss Prot: P68431
Source: KLH conjugated synthetic peptide derived from human Histone H3 (Butyryl-Lys14)
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1ug/ul
Applications: WB(1:500-2000)
Predicted Molecular Weight: 15
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): MCF-7+serum starvation (14 hr), (+): MCF-7+ sodium butyrate (50 mM, 24 hr) + trichostain A (500 ng/ml, 4 hr) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): HeLa, (+): HeLa+ sodium butyrate (30 mM, 4 hr) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Neuro-2a, 2: BRL Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa