bsm-60706R [Primary Antibody]
Alpha Tubulin (Acetyl-Lys40) Recombinant Antibody
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Host: Rabbit

Target Protein: Alpha Tubulin (Acetyl-Lys40)

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 7277

Swiss Prot: P68366

Source: KLH conjugated synthetic peptide derived from human Alpha Tubulin (Acetyl-Lys40)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Size: 100µL

Concentration: 1ug/ul

Predicted Molecular Weight: 50


Predicted Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chengmin Feng. et al. Preparation of healing-promoting and fibrosis-inhibiting asymmetric poly(ethylene glycol-b-L-phenylalanine)/cRGD-modified hyaluronate sponges and their applications in hemorrhage and nasal mucosa repair. INT J BIOL MACROMOL. 2024 Feb;258:128911Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Right temporal lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Tubulin (Acetyl-Lys40) Monoclonal Antibody, Unconjugated (bsm-60706R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Hela (H) cells were treated with or without Trichostatin A (500 ng/ml ) for 4 h, 25 μg total protein per lane of cell lysates (see on figure) probed with Alpha Tubulin (Acetyl-Lys40) monoclonal antibody, unconjugated (bsm-60706R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.