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Host: Rabbit
Target Protein: phospho-IKB Alpha (Ser32 / Ser36)
Modification Site: Ser32 / Ser36
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 4792
Swiss Prot: Q9Z1E3
Source: KLH conjugated synthetic peptide derived from human phospho-IKB Alpha (Ser32 / Ser36)
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: Lot Dependent
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF
Predicted Molecular Weight: 35
Predicted Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Feng Gao. et al. Goat milk exosomal microRNAs alleviate LPS-induced intestinal inflammation in mice. INT J BIOL MACROMOL. 2024 May;268:131698Read more>>
Tissue: (A) Rat kidney, (B) Rat kidney, then the tissue was incubated with alkaline phosphatase (50 U/ml) at 37℃ for 1 hour Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60711R
Tissue: (A) Mouse kidney, (B) Mouse kidney, then the tissue was incubated with alkaline phosphatase (50 U/ml) at 37℃ for 1 hour Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60711R
MCF-7 (H) treated with TNF-alpha (20 ng/mL) for 8 h, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-IKB Alpha (Ser32 / Ser36) monoclonal antibody, unconjugated (bsm-60711R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
C6 (R) treated with Calyculin A (100 ng/mL) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-IKB Alpha (Ser32 / Ser36) monoclonal antibody, unconjugated (bsm-60711R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.