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Host: Rabbit
Target Protein: CD8 alpha
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 925
Swiss Prot: P01732
Source: KLH conjugated synthetic peptide derived from human CD8 alpha
Purification: Purified by Protein A.
Storage Buffer: PBS, Glycerol, BSA.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100uL
Concentration: 1mg/ml
Applications:
WB(WB(1:1000-5000))
IHC-P(IHC-P(1:200-500))
IHC-F(IHC-F(1:200-500))
IF(IHC-P)(IF(IHC-P)(1:200-500))
Predicted Molecular Weight: 27
Cross Reactive Species: Mouse
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Mouse Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8 alpha Monoclonal Antibody, Unconjugated (bsm-60734R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8 alpha Monoclonal Antibody, Unconjugated (bsm-60734R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8 alpha Monoclonal Antibody, Unconjugated (bsm-60734R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD8 alpha Monoclonal Antibody, Unconjugated (bsm-60734R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with CD8 alpha monoclonal antibody, unconjugated (bsm-60734R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.